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matrigel  (World Precision Instruments)


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    World Precision Instruments matrigel
    Matrigel, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 96/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/matrigel/product/World Precision Instruments
    Average 96 stars, based on 303 article reviews
    matrigel - by Bioz Stars, 2026-05
    96/100 stars

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    <t>Erastin</t> treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    <t>Erastin</t> treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Matrigel, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    <t>Erastin</t> treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    <t>Erastin</t> treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    <t>Erastin</t> treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    <t>Erastin</t> treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    <t>Erastin</t> treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Matrigel Pbs, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    <t>Erastin</t> treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Matrigel, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/matrigel/product/Beijing Solarbio Science
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    Image Search Results


    Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Umbilical cord mesenchymal stem cells and their extracellular vesicles attenuate cryopreservation-induced ovarian injury via the suppression of ferroptosis in an in vitro culture system

    doi: 10.1016/j.mtbio.2026.102965

    Figure Lengend Snippet: Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: The culture medium was replenished every 48 h. The cryopreserved ovarian tissues were randomly divided into six groups for in vitro experiments: (1) Control group (Ctrl), freshly isolated ovaries encapsulated in Matrigel; (2) Cryopreservation group (Cryo), frozen-thawed ovaries encapsulated in Matrigel; (3) Erastin group (Ers), freshly isolated ovaries encapsulated in Matrigel with erastin (30 μM; MedChemExpress, USA) during the first 2 days of culture; (4) MSC group, frozen-thawed ovaries encapsulated in Matrigel and cocultured with UC-MSCs for 4 days; (5) Fer-1 group (Fer-1), frozen-thawed ovaries encapsulated in Matrigel with Fer-1 (50 μM; MedChemExpress, USA) during the first 2 days of culture; and (6) Erastin + MSC group (Ers + MSC), freshly isolated ovaries encapsulated in Matrigel, treated with erastin for the first 2 days and subsequently cocultured with UC-MSCs for the remaining 2 days.

    Techniques: Immunohistochemical staining, Staining, Colorimetric Assay, Fluorescence